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Therapeutic Hypothermia Reduces the Inflammatory Response Following Ischemia/Reperfusion Injury in Rat Hearts
Author(s) -
Shi Jianru,
Dai Wangde,
Kloner Robert A
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1277.1
Subject(s) - hypothermia , medicine , ischemia , anesthesia , reperfusion injury , inflammation , cardiology
Hypothermia is known to protect against ischemia/reperfusion (I/R) injury, however, the mechanisms underlying its cardiac protective effect are poorly understood. A major mechanism of I/R injury is the inflammatory response. We therefore hypothesized that therapeutic hypothermia reduces the inflammatory response following I/R injury in rat hearts. Rats were randomized to normothermic or hypothermia groups and subjected to 1 hour coronary artery occlusion and 48 hours reperfusion. Hypothermia was initiated 2 minutes after the onset of coronary artery occlusion to a core temperature of 32°C, and then allowed to rewarm. After 48 hours, blue dye was injected to separate the risk zone and non‐risk zone. Heart samples were collected from shams, Risk/normothermia, Risk/hypothermia, Non‐risk/normothermia and Non‐risk/hypothermia. qRT‐PCR analysis showed that MCP1, IL6, TGFb1 and TNFa gene expression were significantly increased by 90.87 fold, p<0.0001, 177.58 fold, p<0.0001, 17.57 fold, p<0.0001 and 7.34 fold, p<0.0001, respectively, in the Risk/normothermia vs sham. Hypothermia significantly decreased the gene expression of MCP1 (p=0.01) and showed a trend of lowering IL6 (p=0.052), TGFb1 and TNFa (p=0.09) expression levels ( Figure). We conclude that hypothermia decreases inflammation gene expression, which may have contributed to the preservation of cardiac structure and function.qRT‐PCR analysis for MCP1, TGFβ1, TNFα and 116 (n=6 in each group). All data were normalized to β‐actin and presented relative to the sham group. *p<0.05 vs. sham # p<0.05 vs. Risk/normothermia.