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LCT Gene Variant ‐13910 C>T, Dairy Intake, and 25‐Hydroxyvitamin D Plasma Levels: A Mendelian Randomization Study
Author(s) -
Alharbi Ohood,
ElSohemy Ahmed
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.127.6
Subject(s) - genotype , mendelian randomization , vitamin d and neurology , genotyping , medicine , zoology , lactose intolerance , endocrinology , biology , lactose , food science , genetics , gene , genetic variants
This abstract is from the Experimental Biology 2016 Meeting. There is no full text article associated with this abstract published in The FASEB Journal . Background The LCT ‐13910 C>T gene variant is associated with lactose intolerance (LI) in different ethnic groups. Individuals with LI often limit or avoid dairy consumption, a main dietary source of vitamin D in North America, which may lead to inadequate vitamin D intake. However, the association between LCT genotype and circulating 25(OH)‐vitamin D levels is not clear. The objective of this study was to determine the prevalence of genotypes predictive of LI in different ethnic groups living in Canada and to determine whether LCT genotype is associated with dairy intake and 25(OH)D plasma levels. Methods Fasting blood samples were drawn from a total of 1,400 participants aged 20–29 years from the Toronto Nutrigenomics and Health Study for genotyping and plasma 25(OH)D analysis. Dairy intake was assessed using a one‐month, 196‐item Toronto‐modified Willett food frequency questionnaire. The prevalence of the LI gene variant LCT ‐13910C>T was compared using. Using the Mendelian randomization (MR) approach, we used analysis of covariance to determine the association between LCT genotypes and dairy intake, as well as 25(OH)D levels. Results Approximately 33% of Caucasians, 99% of East Asians, 74% of South Asians, and 59% of those with other or mixed ethnicities had the CC genotype predictive of LI. The CC genotype was significantly associated with lower total dairy intake (p=0.001), lower skim milk intake (p=0.002), and lower plasma 25(OH)D (p=0.0004). The CC and the CT genotypes were associated with a two‐fold, and 50% greater risk, respectively, of suboptimal plasma 25(OH)D. Conclusion The CC genotype predictive of LI is associated with lower plasma 25(OH)D, which is due, at least in part, to lower intake of dairy products, particularly skim milk. Increased risk of suboptimal levels of vitamin D was also observed among those with the CT genotype suggesting an intermediate effect of heterozygotes. Support or Funding Information: Research support from the Advanced Foods and Materials Network