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Ramipril could attenuate thioacetamide‐induced liver fibrosis in rats
Author(s) -
Abdelghany Tarek Mohammed Mamdouh,
Abdelsalam Rania Mohsen,
Attia Amina Salem,
Elsayed Mostafa Elsayed
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1267.1
Subject(s) - thioacetamide , hydroxyproline , fibrosis , endocrinology , medicine , ramipril , hepatic fibrosis , chemistry , oxidative stress , blood pressure
Liver fibrosis results from chronic damage to the liver in conjunction with the accumulation of extracellular matrix proteins including collagen which is a characteristic of most types of chronic liver diseases. Thus, the present study aimed to investigate the possible prophylactic and therapeutic effects of an angiotensin converting enzyme inhibitor, ramipril, on liver fibrosis induced by thioacetamide (TAA) in rats. Rats (n=40) were classified into 4 groups (n=10), normal control, fibrotic control and two fibrotic‐treated in which ramipril 2.5 or 5mg/kg, p.o. was given daily for 6 weeks. Liver fibrosis was induced by TAA (200 mg/kg, ip) twice per week for 6 weeks in all groups except in the normal control group. At the end of the 6th week blood samples were withdrawn from orbital sinus for measurement of plasma AST & ALT and animals were sacrificed and then livers were separated & homogenized for measurement of oxidative stress biomarkers (MDA and reduced GSH), inflammatory markers (TNFα, IL10 and MPO) and a fibrotic marker (hydroxyproline). Furthermore, MMP‐13, TIMP‐1, α‐smooth muscle actin and collagen‐1 gene expressions were estimated using RT‐PCR and TGFβ was estimated using western‐plot method. In the fibrotic group plasma aminotransferase activity, MPO activity MDA, TNFα, Hydroxyproline, Collagen‐1, α smooth muscle actin, TIMP‐1 and TGFβ contents increased coupled by decrease in reduced GSH and MMP‐13 contents. Treatment with ramipril reversed most of the measured parameters. The protective effect of the tested agent could be attributed to its anti‐inflammatory, anti‐fibrotic & anti‐oxidant effects.