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Intranasal Neuropeptide Y to Prevent Development of Traumatic Stress Elicited Impairments in Rodent PTSD Model
Author(s) -
Sabban Esther,
Serova Lidia,
Hansson Evelyn
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1265.1
Subject(s) - neuropeptide y receptor , stressor , hypothalamus , locus coeruleus , endocrinology , medicine , behavioural despair test , amygdala , corticotropin releasing hormone , tyrosine hydroxylase , hippocampus , psychology , neuropeptide , neuroscience , dopamine , central nervous system , receptor , antidepressant
Traumatic stress is a major contributor to the development of PTSD, as well as co‐morbid depression and substance abuse. Treatment of PTSD is extremely challenging and there are few effective options. The NPYergic system is associated with resilience or improved recovery from harmful effects of traumatic stress. Using the single prolonged stress (SPS) model of PTSD, a single infusion of 150 μg NPY immediately after the stressors (SPS/NPY), prevented or greatly reduced development of many of the behavioral, neuroendocrine and molecular impairments (Serova et al, 2013, Neuroscience, 236:298–312; Laukova et al., 2014, Endocrinolgy 155: 3920–33; Sabban et al, J. Neurochem, in press). A week or more after exposure to SPS stressors animals given vehicle (SPS/V) had elevated anxiety, depressive‐like behavior and hyperarousal, especially to high decibels, while the NPY treated rats were similar to unstressed controls. Intranasal NPY also prevented changes in hypothalamus, hippocampus and locus coeruleus. However, all the stressed animals (SPS/V and SPS/NPY) had reduced body weight. Therefor we examined feeding behavior and gene expression in arcuate nucleus, which plays an important role in regulation of food intake and energy homeostasis. With unrestricted access to food, SPS treated animals (SPS/V and SPS/NPY) had reduced food intake in the active (dark) phase as much as two days after SPS stressors. SPS led to reduced mRNA levels of NPY, tyrosine hydroxylase and elevated AGRP mRNA in the arcuate nucleus of SPS/V animals a week after SPS stressors, at time of the behavioral impairments. However these changes in gene expression in the arcuate were not observed in NPY treated animals, which had levels similar to controls. These findings further support likely benefits of early pharmacological intervention with intranasal NPY following exposure to traumatic stress. Support or Funding Information US army grant DM102281