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Deep‐sea water administration efficiently inhibits the growth of Helicobacter pylori through the inhibition of caspase‐1/inflammasome/pyroptosis signaling
Author(s) -
KOU WENG SI,
YANG JYH CHIN,
CHIEN CHIANG TING
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1253.2
Subject(s) - pyroptosis , autophagy , inflammasome , apoptosis , helicobacter pylori , caspase 1 , necroptosis , in vivo , gastric mucosa , caspase 3 , chemistry , inflammation , biology , microbiology and biotechnology , immunology , programmed cell death , biochemistry , stomach , genetics
The autophagy, apoptosis and inflammasome/caspase‐1 signaling pathways play a critical role in pathogenesis of H. pylori. Deep‐sea water (DSW) rich in micronutrients and minerals and with antioxidant and anti‐inflammatory qualities may be developed as marine drugs to provide gastric protection and therapeutic potential against H. pylori infection. Here, we investigated the effect of DSW on H. pylori‐induced autophagy, apoptosis and caspase‐1‐mediated epithelial damage, as well as H. pylori colonization in vitro (AGS cells) and in vivo (BALB/c mice). Our results found that H. pylori‐infection activated caspase 3‐induced apoptosis and caspase‐1/IL‐1β mediated pyroptosis and downregulated LC3 II‐regulated autophagy in AGS cells and gastric tissue of mice. In addition, we observed increased oxidative stress, NADPH oxidase gp91phox, CD68, caspase‐1/IL‐1β, and apoptosis, but decreased autophagy, in the gastric mucosa of H. pylori‐infected mice. We have further demonstrated that treatment with DSW led to anti‐H. pylori activity. Treatment with DSW for 14 days eradicated H. pylori infection in H. pylori‐infected mice. Taken together, we suggest that DSW is capable to inhibit H. pylori–activated inflammasome/caspase‐1 signaling, caspase 3‐mediated apoptosis and downregulated LC3 II‐mediated autophagy effectively eradicating H. pylori infection.