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The Impact of Acetaminophen Consumption Prior to Resistance Exercise on the Initial Myogenic Gene Response in Human Skeletal Muscle
Author(s) -
D'Lugos Andrew,
Patel Shivam,
Eldon Erica,
Carroll Chad,
Dickinson Jared
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1245.35
Subject(s) - skeletal muscle , endocrinology , medicine , gene expression , vastus lateralis muscle , myf5 , adaptive response , messenger rna , chemistry , biology , myod , gene , biochemistry , genetics , myogenesis
Resistance exercise is a powerful stimulus for skeletal muscle adaptation. Previous data have shown that cyclooxygenase (COX) inhibiting drugs may interact with the cellular mechanisms regulating the adaptive response of skeletal muscle. The purpose of this study was to determine if prior consumption of the COX‐inhibitor acetaminophen (APAP) alters the immediate myogenic gene response in skeletal muscle following resistance exercise (RE). In a double‐blinded, crossover design, healthy young men (n=4) performed two trials of single‐legged RE (8 sets, 10 reps, 70% max strength). Subjects ingested either APAP (1000mg/6h) or placebo (PLA) for 24h prior to RE (final dose consumed immediately post RE). RT‐qPCR was used to examine mRNA expression of myogenic precursors in skeletal muscle ( vastus lateralis ) biopsies obtained at rest and at 1, and 3h post RE. Relative to basal values, DLK1 mRNA expression was increased at 1h post RE in both groups (P<0.05). VEGFA mRNA expression was increased 1h post RE in both groups, and remained elevated 3h post RE only in APAP (P<0.05). MYOG mRNA expression was increased at 3h post RE only in PLA (P<0.05), whereas MGF mRNA expression was decreased 3h post RE only with APAP (P<0.05). MYF5 , MYF6 , and PAX7 mRNA expression were unchanged in both groups and at all time points (P>0.05). These preliminary data indicate that APAP consumption prior to RE may induce subtle alterations in the immediate myogenic gene response to heavy resistance exercise. These data further highlight a unique interaction between the consumption of COX‐inhibiting drugs and the processes that regulate skeletal muscle adaptations to exercise.