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The renin angiotensin system (RAS) in the heart: Effects of aerobic training
Author(s) -
Silva Sebastiao Donato,
Jara Zaira Palomino,
Moreira Roseli,
Montezano Augusto,
Casarini Dulce,
Touyz Rhian,
Michelini Lisete Compagno
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1239.2
Subject(s) - medicine , endocrinology , muscle hypertrophy , aerobic exercise , ventricle , renin–angiotensin system , angiotensin ii , heart rate , oxidative stress , chemistry , blood pressure
Goals To investigating the sequential effects of aerobic training on cardiac Ang II/Ang (1–7) ratio and its relationship with cardiac hypertrophy and inflammatory markers. Methods Male SHR were trained (T) or kept sedentary (S) for 2 months. After measurement of resting arterial pressure (AP) and heart rate (HR) at weeks 0 (S 0 ), 1 (T 1 ), 2 (T 2 ), 4 (T 4 ) e 8 (S 8 and T 8 ) rats were euthanized for heart harvesting. Ang II and Ang (1–7) (HPLC), oxidative stress (DHE staining) and genic expression of hypertrophy and inflammatory markers were measured in the left ventricle (LV). WKY rats were used as time‐control Results SHR‐S 0 vs. WKY‐S 0 had higher AP and HR (169±1 mmHg; 358±7 bpm), LV hypertrophy (corresponding to increases of +27% in LV/BW and 2.4‐, 3.9‐ and 4.0‐fold in α‐MHC, β‐MHC and α‐actin, respectively), increased ROS density (+19%) and elevated TNFα expression (2.8‐fold) in LV, but similar Ang II and Ang‐(1–7) content (87±3 and 54±3 pmol/g tissue, respectively). In the SHR, T promptly reduced LV Ang II (−71%, T 1 ), increased Ang‐(1–7) (+30%, T 2 ) and reduced and normalized DHE density (T 2 ), with a transitory increase of IL‐1b and TNFα (T 1 ‐T 2 ) and a transitory decrease of β‐MHC (T 1 ) and α‐MHC (T 2 ). LV Ang II/Ang (1–7) ratio reduction (−73%) and normalization of ROS content were maintained up to T 8 , while β‐MHC (T 8 ), IL‐1b and TNFα (T 4 ‐T 8 ) were reduced below S levels. In the SHR‐T, α‐MHC and α‐actin were still higher and LV/BW similar to that of SHR‐S at the end of protocols. T effects were accompanied by HR (−15%, T 4 ‐T 8 ) and AP (−10%, T 8 ) reductions. Conclusion Although not reducing LV hypertrophy of the SHR, training reduces RAS vasoconstrictor, proliferative and trophic axis, improves oxidative stress (early normalization) and reduces the pro‐inflammatory profile and β‐MHC expression (late effects) in the heart. Support or Funding Information Fapesp; Capes; Cnpq