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Monocyte Mitochondrial Function as a Marker of Calcium Oxalate Stone Formers
Author(s) -
Mitchell Tanecia,
Williams Jennifer,
Holmes Ross P,
Assimos Dean G
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1220.6
Subject(s) - bioenergetics , monocyte , calcium oxalate , pathogenesis , inflammation , calcium , oxidative stress , medicine , reactive oxygen species , immune system , immunology , chemistry , endocrinology , mitochondrion , biochemistry
The prevalence of kidney stones (KS) is 8.8% in the US and contributes significantly to health care costs. The most common type of KS is the calcium oxalate kidney stone (CaOx‐KS). Both inflammation and oxidative stress have been suggested to play an integral role in KS pathogenesis. The purpose of this study was to investigate whether inflammation is elevated and cellular bioenergetics is suppressed in immune cells from calcium oxalate (CaOx) stone formers (SF) compared to healthy subjects (HS). Adult age‐matched HS (n=18) and CaOx SF (n=15) were included in the study. Data collection included demographic and clinical values from electronic medical records. Pro‐inflammatory Interleukin‐6 (IL‐6) plasma values were measured using ELISA. Bioenergetic function was assessed in monocytes, lymphocytes, and platelets using the Seahorse XF96 Analyzer. Fifty‐three percent of the CaOx SF had a family history of KS and 60% had a KS event previously. Plasma IL‐6 levels were significantly elevated (p=0.0024) and monocyte mitochondrial function was decreased in CaOx‐SF compared to HS. Specifically, maximal respiration (p=0.0001) and reserve capacity (p<0.0001) were significantly lower. In contrast, lymphocyte and platelet mitochondrial function was similar to monocytes from HS. The bioenergetic health index (BHI), an integrated value of mitochondrial function, was also determined to be significantly lower in CaOx SF monocytes compared to HS (p=0.0191). These data suggest that impaired monocyte mitochondrial function may play a role in CaOx KS pathogenesis and the BHI could potentially serve as a marker in CaOx SF. Support or Funding Information Supported by NIH grants DK054468, UL1TR001417, P30 DK079337, and the 2015–2016 UAB Faculty Development Grant Program (TM). TM is also supported by DK054468‐S1.