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Kidney decapsulation reduces ischemia‐reperfusion‐induced proteinuria
Author(s) -
Nensén Oskar,
Hansell Peter,
Fasching Angelica,
Palm Fredrik
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1217.9
Subject(s) - proteinuria , renal function , kidney , ischemia , urology , medicine , endocrinology , filtration fraction , urinary system , chemistry , renal blood flow
Ischemia‐reperfusion injury (IRI) alters the integrity of the glomerular barrier integrity resulting in proteinuria. Decapsulation reduces interstitial pressure in the kidney; a procedure which we have previously reported to reduce diabetes‐induced glomerular protein leakage. Therefore, the present study determined if the same beneficial effects of decapsulation on proteinuria is achieved during the acute phase following IRI in rats. Measurements of urinary protein leakage, tubular pressures and renal blood flow (RBF) were determined before and immediately after thirty minutes of warm complete unilateral ischemia in normal and decapsulated adult male Sprague Dawley rats. Decapsulation per se completely prevented the IRI‐induced proteinuria. Decapsulation concomitantly reduced both baseline tubular free flow pressure (P ff ; 14.0±0.6 versus 11.9±0.8 mmHg) and tubular stop flow pressure (P sf ; 32.1±0.8 versus 30.2±0.7 mmHg) resulting in similar net filtration pressures (P net ) in both groups (18.1±0.5 and 18.3±0.7 mmHg). IRI resulted in increased P ff in both groups (+5.0±1.0 and +4.9±0.8 mmHg for controls and decapsulated, respectively). P sf was unaltered by IRI resulting in similar reduction in P net after IRI in both groups. Interestingly, decapsulation reduced baseline RBF (7.8±1.1 versus 6.6±0.5 ml/min/kidney) but significantly mitigated the IRI‐induced reduction in RBF (−3.6±1.6 versus −1.9±0.7 ml/min/kidney for control and decapsulated, respectively). These data demonstrate that decapsulation prevents IRI‐induced proteinuria, possibly by reducing interstitial hydrostatic pressure. It is tempting to speculate that therapies lowering renal interstitial pressure could potentially be used to prevent IRI‐induced proteinuria and protect long‐term kidney function.