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The Effects of Captopril Treatment and Exercise and Blood Pressure, Endothelium Function and Gene Expression of the Renin Angiotensin System in the Spontaneously Hypertensive Rat
Author(s) -
Enyinnia Diamond Enyinnia,
Henry Eden,
Okoire Nnaemeka,
Vaden Deirdre,
Keaton Alphonso
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1206.4
Subject(s) - captopril , blood pressure , medicine , endocrinology , isometric exercise , angiotensin converting enzyme , renin–angiotensin system , spontaneously hypertensive rat , endothelium , plasma renin activity , angiotensin ii
Angiotensin converting enzyme inhibitors (ACE‐I) have been show to prevent impairment of endothelial cell function in the Spontaneously Hypertensive Rat (SHR). The purpose of this study was to examine the combined effects of long‐term exercise training and ACE‐I treatment on blood pressure, vascular reactivity and gene expression of the Renin Angiotensin System (RAS), in the SHR. Three groups of male SHR were studied: 1) untreated SHR ; 2) exercise trained (35 cm/s) for 60 minutes/day, for 3 days/week (SHREX) ; 3) exercised trained + captopril treated (50 mg/kg/day), treatment was initiated at four weeks of age and continued for 16 weeks (CAPEX). N = 8, for all three groups. At 20 weeks of age, systolic blood pressure (via tail cuff) in the untreated SHR was 190±11 mmHg. Exercise treatment in the SHR significantly decrease MAP in the SHREX was 151±7 mmHg). The combination of exercise and oral administration of captopril was associated with a significant decrease in systolic blood pressure (130±3 mmHg) compared to untreated SHR and SHREX. Isolated 3mm aortic ring segments were suspended in tissue chambers for measurement of isometric force. Ring segments were exposed to cumulative concentrations of serotonin (3× 10 −9 – 3 × 10 −5 M). Preliminary data from aortic rings from SHR demonstrated increased responsiveness to serotonin induced contraction in comparison to aortic rings from the SHREX and CAPEX group. Endothelium‐dependent relaxation to acetylcholine was dramatically impaired in the SHR. Harvested tissue samples from the brain, kidney liver and blood will be used to examine gene expression of the renin angiotensin system using Next Generation RNA Sequencing. Support or Funding Information Mini Grant Award from the Office of Research and Graduate School, Prairie View A&M University