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Effect of Hibiscus sabdariffa Calyx Extract on Erythrocytic Indices in Sickle Cell Anemia in Vitro
Author(s) -
Mojiminiyi Frank Babatunde,
Pereye Blessing,
Ndakotsu Mohammed,
Ndodo Darlington,
Ikhuenbor Dorcas,
Igbokwe Vincent
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1192.5
Subject(s) - hibiscus sabdariffa , calyx , hemoglobin , chemistry , in vitro , analysis of variance , pharmacology , zoology , medicine , biology , biochemistry , food science , botany
This study tested the antisickling properties of Hibiscus sabdariffa calyx extract (HS) in vitro. Following ethical approval, 5 ml of venous blood was collected from SCA patients and baseline haematological indices were determined. The blood was divided into 4 groups. To the first group, sodium metabisulphite (Na 2 S 2 O 5 ) was added to the blood and then incubated with 0.l, 1 and 10mg/ml of HS (reversal assay). To the second group, the blood was incubated with different concentrations (0.l, 1 and 10mg/ml) of HS extract before adding Na 2 S 2 O 5 , and then haematological indices were determined (protective assay). The third group was incubated with 0.5 mg/ml Hydroxyurea and the fourth with 0.9% NaCl. Experiments were performed in accordance with the Principles of the Declaration of Helsinki. Results are expressed as mean±SEM. Statistical Analyses were done using ANOVA, and a post hoc Dunnett's test. P<0.05 was considered significant. In the protective assay, there was a dose dependent significant decrease in MCHC in the HS treated groups (36.64±0.47g/dL in both 0.1mg/ml and 1mg/ml and 31.57±1.65g/dL 10mg/ml) compared to control (37.76±0.35g/dL; P=0.0017). In the reversal assay, HS had a dose dependent decrease in MCHC (34.98±0.61g/dL, 35.22±0.65g/dL and 33.08±2.08g/dL in 0.1,1 and 10mg/ml respectively) compared to control (37.76±0.35g/dL; P=0.0021). However, HS had no significant effect on MCV, RDW and MCH. These results suggest that HS may have antisickling properties.