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Inhibitory Effect of 4‐hydroxysattacin, Extract of Soil Bacteria, on Melanogenesis Using B16F10 Melanoma Cells And 3D Human Skin Model
Author(s) -
Kim Kyuri,
Park Hyeonji,
Nam Sangjip,
Lim KyungMin
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1192.3
Subject(s) - tyrosinase , melanin , melanocyte , human skin , hyperpigmentation , melanoma , melanosome , melanocyte stimulating hormone , skin hyperpigmentation , chemistry , skin cancer , enzyme , biology , cancer research , biochemistry , hormone , cancer , genetics
Epidermal or dermal pigmentation is a skin disorder produced from accumulated melanins in hyperactivated melanocyte. Various chemicals or natural compounds have been reported as a therapeutic agent of hyperpigmentary disorders that manifests, post‐inflammatory, melanoderma, solar lentigo, or freckles. Here, we investigated melisma inhibitory effect of 4‐hydroxysattabacin, crude extract of soil bacteria, Bacillus sp , on melanogenesis in murine melanoma cell, B16F10, and UVB‐induced hyperpigmentation on 3D‐human skin model. Using alpha‐melanocyte stimulating hormone (a‐MSH) as triggering factor of pigmentation, 4‐hydroxysattabacin treatment decreased melanin contents in dose dependent manner. The Real‐Time Polymerase Chain Reaction result demonstrated 48 hour treatment of 4‐hydroxysattabacin produced down‐regulation of several melanogenic genes that encompass Tyrosinase, Tyrosinase‐related protein 1 (TRP‐1), and Tyrosinase‐related protein 2 (TRP‐2) while melanogenic enzyme activities were slightly affected. Moreover, diminution of Tyrosinase protein expression was confirmed by immunoblotting assay as well as anti‐melanogenic effects of 4‐hydroxysattabacin was proved on 3D human epidermal skin model, which supplant animal test for cosmetic testing. Finally, inhibitory effect of 4‐hydroxysattabacin was clarified both in vitro and 3D human skin model that is to say 4‐hydroxysattabacin would be a potential therapeutic agent for cutaneous hyperpigmentation.