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Protective effect of Pteropyrum scoparium and Oxalis corniculata against streptozotocin‐ induced diabetes in rats
Author(s) -
AlQalhati Iman Rashid Sultan,
Waly Mostafa,
AlAttabi Zahir,
AlSubhi Lyutha Khalfan
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1176.4
Subject(s) - streptozotocin , diabetes mellitus , oxidative stress , medicine , antioxidant , population , intraperitoneal injection , type 2 diabetes , traditional medicine , endocrinology , biology , biochemistry , environmental health
Background Type 2 diabetes represents a major health concern among Omani adults population, and it is perceived as a lifestyle‐related disease. There is a lack of research that identifies the potential anti‐diabetic effects of edible wild Omani plants. Therefore understanding the value and usability of edible wild Omani plants in the food chain of Omani adults might be considered as an effective intervention for the primary prevention of type 2 diabetes. Objective This study aimed to assess the in‐vivo potential anti‐diabetic and antioxidants effects of Pteropyrum scoparium and Oxalis corniculata plants or their mixture. Methods Forty five adult Sprague Dawley rats, weighting 250–300 grams, were allocated as into 5 groups (9 rats/group): non‐diabetic control that received chow diet; diabetic group that received chow diet; and diabetic groups that received chow diet plus oral feeding of Pteropyrum scoparium , Oxalis corniculata extracts or their mixture. Diabetes was induced by a single intraperitoneal injection dose of streptozotocin (STZ) drug, 60 gm/kg body weight. At the end of the experimental trial, after 8 weeks, all rats were fasted overnight and sacrificed; serum was collected for measurement of glucose, meanwhile pancreatic tissues was dissected and homogenized for the biochemical assessment of antioxidant enzyme (superoxide dismutase) and oxidative stress markers (glutathione and total antioxidant capacity). Results Diabetic rats treated with plants extracts had shown a significant protective effect against STZ‐induced hyperglycemia and oxidative stress as compared with the control group. Microscopic examination of pancreatic diabetic treated groups showed no histopathological changes indicating that the treatment with plants extracts or their mixture have no effect on the pancreatic tissues. Conclusion This study demonstrated that Pteropyrum scoparium and Oxalis corniculata combated the STZ‐mediated hyperglycemia and oxidative stress. Histopathological examination revealed that there was no observed effect on the cellular pancreatic moiety, indicating safety of the used plants extracts. Support or Funding Information This research was supported by internal grant funding, IG/AGR/FOOD/14/2, from Sultan Qaboos University