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Effects of a natural extract of Aronia Melanocarpa berry on endothelial cell nitric oxide production
Author(s) -
Fromentin Emilie,
Varela Claudia Elena,
Roller Marc,
Villarreal Francisco,
RamirezSanchez Israel Elena
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1175.13
Subject(s) - enos , nitric oxide , polyphenol , chemistry , endothelial nitric oxide synthase , berry , in vitro , pharmacology , nitric oxide synthase , biochemistry , medicine , botany , biology , antioxidant , organic chemistry
Polyphenol‐rich diets are known to have a positive impact on nitric oxide (NO) production thus, decreasing the risk of cardiovascular diseases. Aronia melanocarpa , also known as black chokeberry, has one of the highest content of phenolic components among various berries. We investigated the effects of acute and chronic treatment with Aronia extracts on NO production and endothelial nitric oxide synthase (eNOS) phosphorylation in bovine coronary artery endothelial cells. Acute time‐course and concentration‐response experiments were performed to determine the time and concentration at which Aronia induced maximal NO synthesis and eNOS phosphorylation. The findings indicate that relatively low concentrations (0.1 μg/mL) of Aronia extract significantly induce NO synthesis and eNOS phosphorylation after 10 min of treatment. An increased sensitivity of eNOS and a significant increase in NO synthesis resulted from longer‐term stimulation with Aronia (48 hr) and an acute re‐treatment of the cells (10 min). These in vitro results may be translated into potential future clinical applications where Aronia extracts may be used for prevention and coadjuvant treatment of cardiovascular diseases via increases in endothelial NO synthesis and related improvements in vascular functions. Given the dose‐response effect of Aronia extract in vitro and metabolism of polyphenols that occurs in humans, dose‐response studies would be necessary to define the optimal daily amount to be consumed. Support or Funding Information This work was funded by NATUREX‐DBS