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The effect of cranberry consumption on lipid and lipoprotein metabolism in human apolipoprotein A‐I transgenic mice fed a high fat and high cholesterol diet
Author(s) -
Park YoungKi,
Caceres Christian,
O'Neill Edward N.,
Bae Minkyung,
Pham Tho X.,
Lee Yoojin,
Kim Bohkyung,
Lee JiYoung
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1175.10
Subject(s) - apolipoprotein b , endocrinology , triglyceride , medicine , cholesterol , chemistry , lipoprotein , lipid metabolism , high density lipoprotein , blood lipids , metabolism , genetically modified mouse , transgene , biology , biochemistry , gene
Cranberries contain high levels of health‐promoting anthocyanins, which have been implicated in their potential role in the prevention of cardiovascular disease. The objective of this study was to determine the effects of cranberries on lipid and lipoprotein metabolism in mice expressing human apolipoprotein A‐I transgene (hApoAI Tg ), which have similar HDL metabolism to that of humans. Male hApoAI Tg mice were fed a modified AIN‐93M high fat/high cholesterol control diet (HF/HC; 15% fat, 0.25% cholesterol, w/w; n = 15) or a HF/HC experimental diet supplemented with 5% (w/w) dried, whole cranberry powder (n = 16) for 8 weeks. Subsequently, the mice were sacrificed to obtain tissue and blood samples. There were no significant differences in terminal body weights, body weight gain, liver weights and epididymal fat weights between control and cranberry‐fed mice. Serum total cholesterol (TC) and apolipoprotein B cholesterol (ApoB‐C) levels were significantly lower in controls than cranberry group (238 ± 9.4 vs. 294 ± 20.3 mg/dL for TC; 43.9 ± 6.7 vs. 88.3 ± 15.0 mg/dL for ApoB‐C) with no significant difference in serum high‐density lipoprotein cholesterol (HDL‐C) concentrations. Serum triglyceride (TG) levels of cranberry group (55.5 ± 4.6 mg/dL) were also significantly higher than controls (37.7 ± 2.9 mg/dL). Hepatic expression of sterol‐regulatory element binding protein 2 and low‐density lipoprotein receptor was significantly lower in cranberry group, which may be responsible for the increased serum TC and apoB‐C, while HMG‐CoA reductase and proprotein convertase subtilisin/kexin type 9 mRNA levels were not different between two groups. Despite the significant increase in serum TG in mice‐fed cranberries, their mRNA abundance of genes responsible for lipogenesis (e.g., fatty acid synthase and stearoyl CoA desaturase) and fatty acid oxidation (e.g., carnitine palmitoyl transferase 1α and acyl‐CoA oxidase 1) was not significantly altered in the liver. In addition, the hepatic expression of genes involved in HDL metabolism, including human apoA‐I, lecithin:cholesterol acyltransferase, and scavenger receptor class B, type I, was significantly lower in cranberry‐fed mice compared with controls although serum HDL‐C levels were not different between two groups. Further study should be warranted to have better mechanistic insight into the changes in lipid and lipoprotein metabolism by cranberries. Support or Funding Information Nutricia Research Foundation