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Protective effect of Korean Diet or American Diet against DNA Damages Induced by H 2 O 2 in Human Lymphocyte
Author(s) -
Lee MinYoung,
Kim HyunA,
Kang MyungHee
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1174.16
Subject(s) - meal , comet assay , medicine , dna damage , damages , zoology , human nutrition , biology , toxicology , food science , dna , biochemistry , political science , law
Korean diet is comprised of mainly vegetables with abundant antioxidants such as vitamins, carotenoids, and phytochemicals compared to American diet. Therefore, Korean diet is expected to have excellent protection against DNA damages. However, limited research studies have been conducted in this area. Therefore, the objective of this study was to compare the protective effect of Korean diet against DNA damages compared to American diet. A standard diet for a week (2,000 Kcal/day) for Koreans provided in 2010 Dietary Reference Intakes for Koreans was used as Korean diet for this study. A diet for a week (2,000 Kcal/day) comprising of food that are generally eaten by Americans based on National Health and Nutrition Examination Survey with the help from the National Institute of Agricultural Sciences of the Korea Rural Development Administration was used as a control group. After cooking and freeze‐dried the components of food in Korean diet and American diet at an individual's intake quantity, diet samples were prepared as one‐time meal, one‐day meal, and one‐week meal. DNA damages were measured using COMET assay after separating lymphocytes from blood of five healthy adults. The protective effect against DNA damages induced by H 2 O 2 was presented as a reduction rate (%) of DNA damage at tail moment. Comparison results for reduction rate of DNA damage per meal randomly selected from Korean diet or American diet showed that the average reduction rate of DNA damage by Korean diet was 77.9 ± 1.7%, which was significantly ( p < 0.01) higher than that by American diet at 69.6 ± 2.0%. For mixed diet (three times of meals and snacks adjusted to meet 2,000 Kcal per day), the reduction rate of DNA damage by Korean diet was 76.5 ± 1.2%, which was also significantly ( p < 0.01) higher than that by American diet at 70.2 ± 1.3%. When the effect of total meals for a week was measured, the reduction rate of DNA damages by Korean diet for a week was 80.5 ± 0.8%, which was significantly ( p < 0.01) higher than that by American diet for a week at 70.6 ± 2.5%). Therefore, the protective effect of Korean diet against DNA damage was superior to that of American diet. Support or Funding Information This research was supported by a grant (NRF‐2013R1A1A3006963) of the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology, Republic of Korea.