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Development of experimental colitis is associated with dysregulation of mineral homeostasis and fecal conditions in rats
Author(s) -
Tsuneki Ikuya,
Iwaya Hitoshi,
Shimizu Hidehisa,
Hara Hiroshi,
Ishizuka Satoshi
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1173.7
Subject(s) - ulcerative colitis , colitis , feces , gastroenterology , inflammatory bowel disease , medicine , bloody , diarrhea , inflammation , body mass index , biology , disease , surgery , microbiology and biotechnology
Ulcerative colitis (UC) is chronic inflammation in large intestine and the development of colitis is considered to be related with dietary factors and microbial environment. UC patients often suffer from symptoms such as diarrhea, bloody stool, frequent defecation and body weight loss. Dextran sulfate sodium (DSS) is used to induce the UC‐like symtoms in experimental animals. To prevent colitis with dietary intervention, we investigated appropriate end point and/or markers in DSS‐treated conditions, espesially in an early phase of the diseaase development. Fischer 344/Jcl male rats (four weeks old) were fed an AIN‐93G‐based diet with or without 1% DSS treatment for 10 days. Mineral levels in aorta plasma were measured by inductively coupled plasma‐mass spectrometry. Changes in the number of feces and appearence were scored every day. Conditions of the colitis were evaluated by disease activity index (DAI) and inflammation‐related gene expressions. Development of DSS colitis was confirmed in DSS‐tretaed rats according to the DAI and upregulation of tnfa , il6, and mcp1 expression in the colonic mucosa. There was a strong correlation among DAI, plasma Ca concentration, and the number of feces. Multivariate analysis revealed that not only plasma Ca but also Fe and Zn are also predictors for DAI. The number of feces as well as plasma mineral concentrations might be useful marker to identify appropriate end point to evaluate the effect of dietary intervention in DSS colitis.