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Anti‐inflammatory Activity of Stearidonic Acid through Suppression of NFκB Activation in RAW264.7 macrophages
Author(s) -
NOH SEUNGWOO,
SUNG JEEHYE,
JEON JIYOUNG,
LEE JAEMIN,
LEE JUNSOO
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1163.20
Subject(s) - nitric oxide , docosahexaenoic acid , inflammation , eicosapentaenoic acid , pharmacology , nitric oxide synthase , lipopolysaccharide , chemistry , anti inflammatory , biochemistry , immunology , biology , fatty acid , polyunsaturated fatty acid , organic chemistry
Inflammation is a crucial body defense mechanism against stimuli such as pathogen invasion or endotoxin exposure. However, excessive or aberrant inflammatory responses contribute to the pathogenesis of various chronic inflammatory and autoimmune diseases such as rheumatoid arthritis, cardiovascular disease, and Alzhehimer's disease. Docosahexaenoic acid (DHA, 22:6n‐3) and eicosapentaenoic acid (EPA, 20:5n‐3) have been found to possess beneficial effects in cardioprotection, anti‐inflammation, vascular disease prevention, metastatic breast cancer incidence reduction, possible type 2 diabetes therapy, and multiple sclerosis. Stearidonic acid (SDA, 18:4n‐3) is one of omega‐3 fatty acids and found in variable amounts in several species including algae, fungi and animal tissues, but the seeds of some plant families seem to be better sources of SDA, especially Echium ( Boraginaceae ) species. To our knowledge, anti‐inflammatory effect of SDA has not yet been reported. In this study, we demonstrated that SDA exert anti‐inflammatory activities in lipopolysaccharide (LPS)‐stimulated RAW264.7 macrophages by the suppression of nuclear factor κB (NFκB) activation. SDA significantly inhibited inducible nitric oxide synthase (iNOS) expression, leading to the suppression of iNOS‐derived nitric oxide (NO) production. These inhibitory effects of SDA were accompanied by decreases in LPS‐induced translocation of NFκB. The results indicate that SDA was a potent inhibitor of NO and iNOS expression in LPS‐stimulated RAW264.7 macrophages. These findings indicate that SDA are potential therapeutic agents for the treatment of inflammatory diseases.