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The Influence of Dietary Fat and Vitamin D on Adiposity and Vitamin D Metabolism in Older Female Mice
Author(s) -
Wang Yang,
Miller Joshua W,
Shapses Sue A
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1163.15
Subject(s) - endocrinology , medicine , vitamin , vitamin d and neurology , obesity , vitamin d deficiency , chemistry , biology
Obesity induced by high fat diets (HFDs) has been shown to be inversely associated with vitamin D status in both humans and rodents. However, it is unclear whether obesity causes reduced vitamin D status, or if vitamin D deficiency is contributing to body adiposity. Additionally, whether different types of fatty acids have specific effects on vitamin D metabolism has not been addressed. In this study, we examined 35 eight‐month‐old female C57BL/6J mice that were fed one of five diets including a normal fat diet (NFD; 10% fat energy) and a HFD (45% fat) that was either enriched with monounsaturated fatty acids (MUFAs) or with saturated fatty acids (SFAs) all containing standard vitamin D levels (1000 IU/kg diet). Additionally, there were two other HFD‐SFA diets with either a high (10,000 IU/kg diet) or low (100 IU/kg diet) level of vitamin D. Mice were analyzed for body composition, hepatic lipid, serum 25‐hydroxycholecalciferol (25(OH)D) concentration, and protein expression of major vitamin D hydroxylases. After 10wk of ad libitum feeding, the HFDs resulted in greater energy intake, weight gain, total body fat and liver fat; and lower serum 25(OH)D than the NFD group ( P < 0.05). There was no differential effect due to the type of fatty acid consumed (SFAs vs. MUFAs), but low vitamin D intake exacerbated adiposity during high fat feeding. It is concluded that the combination of low dietary vitamin D and high fat intake leads to excessive adiposity. However, supplemental vitamin D has no beneficial effects on preventing HFD‐induced obesity. Support or Funding Information NIH‐AG12161 (SAS) and Rutgers Univ. School of Environmental & Biological Sciences (JWM)