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Anti‐inflammatory effect of glucose‐lysine Mallard reaction products ameliorate on dextran sulfate sodium‐induced colitis in rats
Author(s) -
Lee KwangWon,
Hong ChungOui
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1148.1
Subject(s) - colitis , inflammatory bowel disease , ulcerative colitis , superoxide dismutase , myeloperoxidase , glutathione peroxidase , pharmacology , chemistry , glutathione , inflammation , medicine , immunology , antioxidant , biochemistry , enzyme , disease
Inflammatory bowel diseases (IBD) such as Crohn's disease and ulcerative colitis are chronic relapsing disorders of the gastrointestinal tract that are charac‐terized pathologically by intestinal inflammation and epithelial injury. The currently most therapies are used like as antibiotics, immunosuppressant and anti‐inflammatory drugs, but side effects and loss of response limit long term effectiveness. To find the effective therapy for IBD patients we evaluate the impact of glucose lysine Maillard reaction products (Glc‐Lys MRPs) on colitis in this study. Colitis was induced in rats by administration of 5% dextran sulfate sodium (DSS) in drinking water. DSS has been used as a representative experimental colitis model, which exhibits several clinical and histopathological features similar to human colitis. The Glc‐Lys MRPs contribute in ameliorating DSS‐induced colitis, determined by decreasing disease index activity (DAI), colon weight/length ratio and nitric oxide (NO) in serum and increasing recovery of body weight loss, colon length and lysozyme in serum. Furthermore, Glc‐Lys MRPs improved glutathione (GSH) content and glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) enzyme activities and inhibited lipid peroxidation (MDA) formation and myeloperoxidase (MPO) enzyme activity in colon tissues. Especially, Glc‐Lys MRPs suppressed inflammatory cytokines mRNA level of tumor necrosis factor‐α (TNF‐α), interleukin‐1β (IL‐1β), IL‐6, IL‐10 and nuclear factor‐κB (NF‐κB) in colon tissues. This study thought that potential of the Glc‐Lys MRPs may play an important role in prevent or therapy on IBD. 1Effect of Glc‐Lys MRPs on relative body weight and disease activity index (DAI) in DSS‐induced colitis on rats.2 Effect of Glc‐Lys MRPs on colonic length and relative colonic weight/length ratio in DSS‐induced colitis on ratsA Macroscorpic appearance, B colon length, and C colon weight/length (grams per centimeter).3Effect of Glc‐Lys MRPs on NO level and lysozyme activity in serum of DSS‐induced colitis on rats.4Effect of Glc‐Lys MRPs on histopathological evaluation in DSS‐induced colitis on rats.5Effect of Glc‐Lys MRPs on activity of myeloperoxidase (MPO) in DSS‐induced colitis on rats.6Inhibitory effect of Glc‐Lys MRPs on inflammatory cytokine mRNA expression in DSS‐induced colitis on rats.