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Overexpression of basonuclin 2 promotes carcinogenesis in basal cell carcinoma
Author(s) -
Douhne Timothy,
Cornett Jonathan C
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1108.17
Subject(s) - carcinogenesis , basal cell carcinoma , biology , cancer research , keratinocyte , cell , oncogene , basal (medicine) , human skin , microbiology and biotechnology , cancer , cell culture , basal cell , pathology , genetics , medicine , cell cycle , endocrinology , insulin
Basal cell carcinoma (BCC) is the most common form of human skin cancer. To identify driver genes in BCC tumorigenesis, we carried out a piggyBac transposon mutagenesis screen in mice. Basonuclin 2 (Bnc2) was identified as a gene that, when overexpressed in the skin, likely contributes to the formation of basal cell carcinomas. However, the mechanism by which Bnc2 acts to promote tumor formation is unknown. To gain insight into its role in normal cells, we overexpressed Bnc2 in human keratinocytes. Bnc2 overexpressing cells showed increased viability compared with control cells. In addition, Bnc2 overexpression caused reduced expression of differentiation markers in human keratinocytes. Taken together, our results suggest that Bnc2 acts as a novel oncogene in BCC progression by preventing differentiation and cell death in Bnc2 overexpressing keratinocytes. Support or Funding Information This work was supported by a Ledford scholarship from the Appalachian College Association.