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Effects of in vivo knockdown of a Cold‐Inducible RNA Binding Protein (CIRP) on Breast Tumorigenesis
Author(s) -
Ochoa Joey L,
Lujan Daniel A,
Peterson Melanie N,
Hathaway Helen J,
Hartley Rebecca S
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1108.15
Subject(s) - gene knockdown , carcinogenesis , cancer research , transfection , biology , metastasis , mammary tumor , rna binding protein , breast cancer , cell growth , downregulation and upregulation , cell culture , rna , microbiology and biotechnology , cancer , gene , genetics
RNA binding proteins (RBPs) are essential to processes that involve RNAs, beginning from their transcription through to their degradation. In this study, we utilize the polyomavirus middle T antigen (PyMT) mouse model to evaluate the role of cold‐inducible RBP (CIRP) in breast tumorigenesis. When PyMT is expressed in the mammary epithelium of mice, it causes aggressive tumorigenesis. CIRP has been shown to incite the translation of mRNAs encoding stress‐induced proteins, and is observed to have increased expression in breast cancer. We have shown that human CIRP overexpression in the PyMT mouse appears to inhibit breast tumorigenesis during early and late stages. Based on these results, the current study set out to evaluate the effects of CIRP knockdown on tumor development. We hypothesize that CIRP knockdown will allow greater progression of tumor growth, as well as a higher rate of metastasis. To address this hypothesis, CIRP was knocked down using siRNA in a PyMT ‐mammary tumor‐derived cell line. PyMT cells transiently transfected with siCIRP and siControl were injected into the mammary fat pad of a wild type mouse, and tumor growth assessed. CIRP knockdown in PyMT cells is being analyzed via RT‐qPCR and western blotting to determine the effective timing tor mammary fat pad injections. Tumor growth kinetics, final tumor burden and metastasis will be determined, as will expression of CIRP targets, proliferation, apoptosis and histopathological analysis. The results of this study will tell us if CIRP overexpression has the potential to protect against breast cancer. Support or Funding Information Initiatives to Maximize Student Development (IMSD GM‐0602010) Grant from the UNM‐HSC allocation Committee (RAC)