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The Role of Semen‐Derived Exosomes on Acute HIV Infection in T Lymphocytes
Author(s) -
Moricz Bridget,
Madison M. Nia,
Welch Jennifer,
Okeoma Chioma
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1103.1
Subject(s) - microvesicles , semen , human immunodeficiency virus (hiv) , biology , virology , reverse transcriptase , viral replication , immunology , jurkat cells , rna , microrna , immune system , t cell , virus , genetics , gene
About 35 million people are infected with HIV and even with antiretroviral therapy, the infected individuals are still not living a normal life expectancy. Exosomes, nanovesicles with host proteins and small RNAs, have been shown to possess antiviral properties including suppression of HIV infection. However, these studies were performed in a short‐term setting within 24–48 hours post infection. Here we sought to determine the role of semen exosomes on HIV infection in a longer setting, up to ten days post infection. Semen exosomes were added to acutely infected Jurkat cells and aliquots of the cells and media were taken every two days. The samples were then analyzed for viral integration, replication and fitness either by qPCR, gel electrophoresis or reverse transcriptase assays. Based on the results, exosomes decrease HIV integration, replication and progeny fitness. Support or Funding Information NSF REU in Microbiology at The University of Iowa through grant number DBI‐1262222