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The C. albicans adenylyl cyclase, CYR1p, binds bacterial cell wall fragments via its LRR domain
Author(s) -
Burch Jason,
Schaefer Amy,
Lauro Mackezie,
Melnyk James,
Glowala Jakub,
Wykoff Dennis,
Grimes Catherine Leimkuhler
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1096.3
Subject(s) - adenylyl cyclase , candida albicans , peptidoglycan , pattern recognition receptor , biology , muramyl dipeptide , pathogen associated molecular pattern , microbiology and biotechnology , receptor , innate immune system , chemistry , biochemistry , cell wall , in vitro
Every organism, from the complex to the single cellular, is surrounded by trillions of microorganisms. In order to survive an organism must be able to recognize and adapt to the microbiome that surrounds it. The ability to recognize these microorganisms occurs when the appropriate pathogen associated molecular pattern (PAMPs) binds to the proper pattern recognition receptor (PRR). PAMPs are molecules associate with microorganisms such as lipopolysaccharide, viral RNA, and peptidoglycan. Interestingly, yeast respond to small molecules generated by bacteria. Recognition of the bacterial cell wall fragment muramyl dipeptide (MDP) by the adenylyl cyclase, CYR1p, triggers C. albicans to enter pathogenic hyphal growth. While not characterized as a PRR, CYR1p contains a Leucine Rich Repeat (LRR) domain, which is a common protein motif found in many PRRs, including toll‐like receptors and nod‐like receptors in human, and is often the site of PAMP binding. Using a surface plasmon resonance (SPR) assay, we were able to determine the binding affinity for MDP to the LRR domain of CYR1p. Similar SPR assays were used to determine which portions of MDP is necessary for binding CYR1p. The molecules found to bind CYR1p will be tested in vivo , to determine which, if any, are able to trigger hyphal growth in C. albicans.