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Glycome Sequencing; Are We There Yet?
Author(s) -
REINHOLD VER NYE
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1095.2
Subject(s) - glycan , glycome , computational biology , sequence (biology) , dna sequencing , chemistry , biology , biochemistry , glycoprotein , gene
Sequence challenges provided by the uncoded glycomes of proteins and lipids are, in principle, comparable to the glycosaminoglycans and proteoglycans. But, in neither case, are there protocols or technologies comparable to those of genes and proteins. But the demands of personalized medicine and the early successes of carbohydrate antibodies and vaccines strongly suggest this is a research limitation that must be addressed soon. Described in this brief presentation is an overview of a “bottoms‐up” sequencing approach that extends from the glycans of proteins and lipids to the glycosaminoglycans. After sample preparation all sequencing is dependent on ion fragmentation and spatial resolution in the ion trap (MS n ). Here two fundamental fragment ions are basic, the common B‐ion and the more elusive A‐ion. This latter ion is time dependent and is generated by combinations of electron rearrangement and group elimination which importantly varies with linkage point. Such detailed accounting provides a comprehensive spatially acquired sequence. Support or Funding Information PEG; Common Fund