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IN VITRO EVALUATION OF CYTOTOXIC AND ANTIOXIDANT ACTIVITY OF ESSENTIAL OIL AND TERPENES OF Satureja macrostema
Author(s) -
SalgadoGarciglia Rafael,
TorresMartínez Rafael,
HernándezGarcía Alejandra,
SaavedraMolina Alfredo,
LópezMeza Joel Edmundo,
OchoaZarzosa Alejandra
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1090.8
Subject(s) - pulegone , menthone , terpene , thymol , dpph , essential oil , chemistry , abts , linalool , linalyl acetate , limonene , satureja , geranyl acetate , food science , antioxidant , organic chemistry
Satureja macrostema is a Mexican medicinal plant known as nurhitini té or nurite, whose antioxidant properties are mainly attributed to the volatile compounds from aerial part. Therefore, the goal of the present study was to investigate the in vitro cytotoxic and antioxidant potential of essential oil and some related terpenes of S. macrostema . Essential oil from fresh aerial parts of S. macrostema plants were prepared by hydrodistillation and characterized by GC‐MS, finding that the major terpenes were caryophyllene, limonene, linalool, menthone, pulegone and thymol. The effects of essential oil and terpenes from S. macrostema on bovine umbilical vein endothelial cells were determined. Different concentrations of essential oil and terpenes (1, 10, 100, 1000 μM) were added to cultured cells and incubated for 24 h. Cell survival was evaluated using the MTT‐based cytotoxicity assay. The essential oil and linalool, menthone and pulegone did not affect the BUVEC viability (> 86%), while limonene, caryophyllene and thymol showed toxicity at 1000 μM, causing less than 60% viability. Nitric oxide (NO) was determined using Griess reaction method in BUVEC induced with Staphylococcus aureus and treated with essential oil and terpenes from S. macrostema. The essential oil and terpenes at 1, 10 and 100 μM showed a diminution of NO production, indicative of the antioxidant activity. In addition, antioxidant effectiveness of essential oil and terpenes was examined by two different radical scavenging methods, DPPH (2,2‐Diphenyl‐1‐picrylhydrazyl) and ABTS (2,2′‐azino‐bis‐3‐ethylbenzthiazoline‐6‐sulfonic acid), testing 1, 10, 100 and 1000 μM. The essential oil showed the highest antioxidant capacity, reaching 53.10% capture of radicals in the DPPH and 92.13% in the ABTS method. Thymol was the terpene with higher antioxidant activity (94%) at 1 μM determined with both methods. The results obtained show that essential oil and the major terpenes of S. macrostema have antioxidant activity, at concentrations that do not compromise BUVEC viability. Support or Funding Information Financial support grant from CONACYT (RTM) and CIC/UMSNH (Project 2.10 to RSG and 14.1 to AOZ).