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Concentration Effects in Identifying Unclumping Reagents
Author(s) -
Zem Gregory,
Mikaelian E.,
Quis L.,
Nwokochah C.,
Osadi F.,
Arana D.,
Barsigian S.,
Dugyawi J.,
Alvarado B.,
Hovakemian G.,
Lam J.,
Eskander M.,
Voldiner M.,
Garcia S.,
Nair D.,
Song J.,
Tashdjian G.,
Nazer S.,
Rashidizand S.,
Constantino G.,
Pilikian N.,
Faretta A.,
Gekchyan H.,
Corscadden L.,
Kelvani M,
DeGuzman K.,
Canta R.,
Korkounian S.,
Strelnicova A.,
Turdjian M.,
Lay M,
Darmali A.,
Memarian N,
Simonyan K.,
Oghlian L.,
Oppenheimer S. B.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1089.1
Subject(s) - reagent , borax , boric acid , polyethylene glycol , chemistry , fixative , chromatography , biochemistry , organic chemistry , raw material , cytoplasm
This study is part of a multi‐year effort to identify anti cell clumping reagents of potential usefulness in anti‐thrombocytic, anti‐cancer and anti‐infection applications. Here using a fixed (Prefer fixative, Anatech Ltd) yeast ( Saccharomyces cerevisiae) model in deionized water, the effects of 3 concentrations (0.1M range) of 3 reagents (boric acid, polyethylene glycol and borax) were tested on their ability to unclump yeast as measured by percentage of single cells present over time (0 time, 20, 40, 60 min) versus controls in the absence of reagents. After 60 min based on 2700 experiments, the mean percent single cells compared with controls without reagents, for the 3 concentrations were: for boric acid +9, +16, +17; for polyethylene glycol +0.5, −2.5. −3.0; for borax −2.6, −0.7, +1.3. Boric acid was the only moderate unclumper identified here. In a typical set of 30 experiments with boric acid, the spread between control and experimental values increased substantially as time increased at reagent concentrations of 3, 6 and 9 mg reagent per ml of deionized water. By 60 min percent singles in experimentals and controls differed by about 70 percent with p values ranging from 0.0003 to 0.6 describing the significance of the spread. This study provides a precise evaluation of the effects of concentration in this model system suggesting that concentration may not substantially influence whether a reagent is an unclumper or not, under the conditions of the relatively high reagent concentrations and the specific protocol used here (support: Sidney Stern Memorial Trust, Cal State contributions from NSF Presidential Award 0731633 and various NIH and NSF grants).