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Accurate Quantification of Choline and Ethanolamine Plasmalogen Molecular Species by Liquid Chromatography coupled with Tandem Mass Spectrometry
Author(s) -
Otoki Yurika,
Kato Shunji,
Kimura Fumiko,
Furukawa Katshutoshi,
Arai Hiroyuki,
Nakagawa Kiyotaka,
Miyazawa Teruo
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1082.7
Subject(s) - chemistry , chromatography , tandem mass spectrometry , quadrupole time of flight , plasmalogen , mass spectrometry , liquid chromatography–mass spectrometry , ethanolamine , phospholipid , organic chemistry , biochemistry , membrane
Tandem mass spectrometry (MS/MS) has been used for the analysis of plasmalogens (Pls), a physiologically important class of vinyl ether‐linked phospholipid. However, MS/MS generally causes little fragmentation of Pls, especially choline Pls (PC‐Pls). Previous MS/MS studies reported increased formation of product ions of PC‐Pls (and ethanolamine Pls (PE‐Pls)) in the presence of ‘alkali metals.’ Therefore, the use of alkali metals may considerably improve the analytical method for the analysis of both PC‐ and PE‐Pls. This was evaluated in the present study using quadrupole‐time‐of‐flight MS/MS and liquid chromatography (LC) coupled with MS/MS. Results from MS/MS confirmed that alkali metals (e.g., sodium) produced significant fragmentation of PC‐Pls and PE‐Pls. A number of structure‐diagnostic product ions exhibiting high intensities were observed under optimized MS/MS conditions using alkali metals. Moreover the ability to selectively and sensitively quantify both PC‐Pls and PE‐Pls at the molecular species level in biological samples (e.g., blood plasma from healthy and Alzheimer's disease subjects) was demonstrated using LC‐MS/MS. The herein developed method appears to be a powerful tool for analyzing Pls and may provide a better understanding of their physiological roles in vivo.