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Characterizing heterogeneity of Cancer Stem Cells Using Raman Microscopy
Author(s) -
Zhang Hua,
Xu Fei,
He Lili,
Xiao Hang
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1082.13
Subject(s) - raman spectroscopy , cancer stem cell , stem cell , cancer cell , chemistry , biophysics , cancer , biology , microbiology and biotechnology , optics , genetics , physics
Characterization of cancer stem cells (CSC) is challenging because of their wide heterogeneity. A detailed understanding of the CSC heterogeneity may contribute to better understanding of the biological significance of CSC. However, practical analytical tools for characterizing cellular heterogeneity of CSC are lacking. Raman spectroscopy is a molecular vibrational spectroscopy can measure chemical signatures of a single cell noninvasively. Herein, we demonstrated the application of Raman Spectroscopy to characterize CSC heterogeneity. The CSC were enriched by tumor sphere formation assay, and fractioned according to the difference in density. Different fractions of cells were analyzed by Raman Spectroscopy, and their heterogeneity were analyzed by principal component analysis (PCA). The results demonstrated that cells with different density showed distinct Raman spectra that reflected difference in biochemical compositions of the cells. The biochemical profiles of CSC are much more heterogeneous compared to those of the bulk cancer cells. Moreover, extreme limiting dilution analysis (ELDA) showed that CSC with certain Raman spectrum pattern could generate more tumor sphere thus higher stemness. Our findings demonstrated that Raman microscopy offers a reliable method for characterizing individual cancer stem cells for their heterogeneity, and further studies are warranted to correlate the Raman spectrum pattern of individual cancer stem cells with their biological significance. Support or Funding Information This study was partially supported by the fund from USDA.Percoll gradient centrifugation study.Different stem cell Raman spectra and normal cell spectra.