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Purification and Characterization of DNA from Fresh Versus Frozen Chicken Embryos
Author(s) -
Byk Jennifer,
Smith Sheila R,
Benore Marilee Ann
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1082.12
Subject(s) - dna , embryo , yield (engineering) , biology , chemistry , biochemistry , microbiology and biotechnology , materials science , metallurgy
Purification of DNA from tissues can be carried out by several methods. The ease of purification, yield and functionality of the DNA isolated varies depending on the method used. Using both frozen and fresh chick embryos, we tested several kits and traditional methods. Comparative analysis of the yields, purity, and integrity of fresh tissue versus frozen tissue were measured. Support or Funding Information University of Michigan Dearborn Campus Grants