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Using Polymerase Chain Reaction Inhibition to Study Small Molecule Binding to DNA
Author(s) -
Ghannam Ryan,
Smith Sheila R,
Benore Marilee Ann
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1082.11
Subject(s) - dna , polymerase chain reaction , polymerase , chemistry , small molecule , inverse polymerase chain reaction , plasmid , molecule , dna polymerase , microbiology and biotechnology , biophysics , computational biology , biology , biochemistry , nested polymerase chain reaction , gene , organic chemistry
Detection of DNA‐molecule interaction can be challenging. Knowing that Polymerase Chain Reaction (PCR) can be inhibited by interfering agents, this interaction was exploited as a potential assay. Using whole plasmid and DNA fragments, the ability of small molecules to inhibit the PCR reaction was studied under diverse conditions and testing several potential inhibitors. Support or Funding Information University of MI Dearborn campus grants