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Proteomic Analysis of Ewing's Sarcoma Cell Lines
Author(s) -
Thompson Rhese,
Kruchten Anne
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1081.7
Subject(s) - sarcoma , cortactin , cancer research , ewing's sarcoma , kinase , cancer , phosphorylation , biology , cancer cell , metastasis , medicine , cell , pathology , microbiology and biotechnology , genetics , cytoskeleton
Ewing's Sarcoma is a pediatric bone cancer that affects approximately 3 in 1,000,000 children each year. The prognosis for a Ewing's patient can be very poor due to the extremely aggressive and metastatic nature of Ewing's Sarcoma cells. Treatment for patients with this cancer typically includes surgery, radiation, and/or chemotherapy. EWS‐Fli1, the chromosomal translocation product responsible for the development of Ewing's Sarcoma, has been shown to upregulate the transcription of the Aurora kinases, a group of serine kinases. The actin‐binding protein cortactin is an actin‐binding protein that has been shown to be involved in cellular migration and metastases in cancer, and its phosphorylation is expected to be of importance in Ewing's Sarcoma metastases. Here we report on the proteomic analysis of Ewing's Sarcoma cell lines RD‐ES‐1 and SK‐ES‐1. We have used 2‐D gel electrophoresis to analyze the phosphorylation states of cortactin in these cells, as well as the overall proteomic patterns of Ewing's Sarcoma cells in response to various treatments. These results provide an increased understanding of protein modification states in metastatic cells, and future experiments will focus on identifying the protein modifications using mass spectrophotometry.