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Host urinary proteins changed significantly in mice infected with malaria
Author(s) -
Li Xundou,
Deng Weiwei,
Wang Heng,
Gao Youhe
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1081.6
Subject(s) - urine , plasmodium yoelii , biomarker , urinary system , western blot , malaria , biomarker discovery , gel electrophoresis , biology , parasite hosting , plasmodium falciparum , blood proteins , blot , difference gel electrophoresis , microbiology and biotechnology , immunology , chemistry , proteomics , biochemistry , parasitemia , endocrinology , gene , world wide web , computer science
Malaria is one of the most prevalent infectious diseases in tropical and subtropical regions. Effective biomarkers are urgently needed. Biomarker is measurable changes associated with physiological and/or pathophysiological conditions. Urine can be better source for biomarker discovery because it accumulates the changes of body while body removes them from blood by homeostatic mechanisms. In this study, we attempted to hunt for urinary biomarkers for malaria using mice infected with Plasmodium yoelii . The urine of mice was collected before infection and 3, 5 days after infection, separated by SDS PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), digested in‐gel based on molecular weight and identified using nanoLC‐MS/MS (liquid chromatography‐mass spectrometry). More than 70% of red blood cells were damaged by plasmodium 5 days after infection so it is expected that there might be big changes in urine. Unexpectedly, no reliable parasite proteins were identified, indicating that extrinsic protein may be mostly degraded in blood rather than draining into the urine. 427 urinary proteins of mice were identified and 22 proteins were significantly changed (n=3; p <0.05; and fold change>2) and 19 of those proteins were increased and 3 were decreased after infection. Some of these proteins participate in the defense against parasites and the regulation of tissue inflammation. CHIA and CLCA1 were further validated by western blot in another eight mice. Urinary peptidome might be worth more attention because in which fragments of parasite proteins may be found there if they exist. As a result, although there are no parasite proteins identified in the urine, the host (mice) urine proteins may be fairly good biomarker candidates for malaria. Support or Funding Information This work was supported by the National Basic Research Program of China (2012CB517606, 2013CB530805, 2014CBA02005 and 2013FY114100).