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Analysis of the expression of the Basigin subset of the immunoglobulin superfamily in mouse brain
Author(s) -
Hall Tavia N,
Ochrietor Judith D
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1081.4
Subject(s) - basigin , immunoglobulin superfamily , immunoglobulin domain , gene , biology , transmembrane protein , complementary dna , microbiology and biotechnology , gene expression , messenger rna , transmembrane domain , function (biology) , genetics , receptor , matrix metalloproteinase
Basigin gene products are members of the immunoglobulin (Ig) superfamily and are structurally related to immunoglobulin molecules. Basigin protein possesses two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic tail, whereas the splice variant Basigin‐2 protein possesses three extracellular Ig domains, a single transmembrane domain, and a cytoplasmic tail. There are two other members of the Basigin subset of the Ig superfamily – the Neuroplastins and Embigin. Neuroplastin gp55 and Embigin are similar in structure to Basigin and Neuroplastin gp65 is similar in structure to Basigin‐2. All five of these proteins are cell adhesion molecules within neural tissues and play various roles in development, structure, and function of those tissues. The purpose of the present study was to analyze the expression of the Basigins, the Neuroplastins, and Embigin during the development of the mouse brain. Mouse brains were obtained using an accepted protocol and RNA was isolated for use in quantitative reverse transcription polymerase chain reaction (q‐RT‐PCR) using primer sets specific for Basigin gene products, Neuroplastin gene products, and Embigin. The values obtained were normalized to 18s rRNA. It was determined that Neuroplastin gene product expression is ten times greater than that of Basigin or Embigin. Their relative expression levels remain the same throughout the life of the mouse. These data suggest that Neuroplastin gene products have a more prominent role in the structure and function of the mouse brain than the other two members of the subset of the Ig superfamily. Support or Funding Information University of North Florida Office of Undergraduate Studies and the Office of Academic Affairs