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Electrochemical‐Aptamer Biosensor Against ENOX2 for Cancer Diagnostics
Author(s) -
Fetter Lisa C,
Bonham Andrew J
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1081.3
Subject(s) - aptamer , biosensor , oligonucleotide , chemistry , cancer biomarkers , systematic evolution of ligands by exponential enrichment , nanotechnology , biochemistry , computational biology , cancer , combinatorial chemistry , microbiology and biotechnology , biology , materials science , rna , dna , genetics , gene
Human Ecto‐Nicotinamide Dinucleotide Oxidase Disulfide Thiol Exchanger 2 (ENOX2), is common isoform of a parent protein ubiquitously expressed in humans, ENOX1. The ENOX2 isoform is only highly expressed on the surface of tumors, and as such has been heavily researched as a potential diagnostic biomarker for cancer. Since ENOX2 is shed into the sera and its presence is cancer‐specific, ENOX2 is a highly attractive target for cancer‐specific diagnostic blood testing. To enable a robust sensing platform that can detect ENOX2 in a rapid, reagentless manner, we have created an aptamer‐scaffold electrochemical bionsensor directed against ENOX2. This project involved identification of an ENOX2‐selective aptamer sequence through SELEX procedures, followed by modifications of the aptamer to support a conformation‐switching response to target binding. This oligonucleotide was then incorporated into an electrode‐based electrochemical biosensor platform, creating a dose‐responsive readout for the presence of ENOX2 based on electrical current changes. Ultimately, this ENOX2 biosensor will function in whole blood and yield a novel, rapid, and clinically‐relevant diagnostic tool for early cancer detection.