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Effect of over the counter teeth bleaching products on the proteins of human teeth
Author(s) -
Swanson Derek,
Keenan Kelly
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1081.2
Subject(s) - enamel paint , dentin , chemistry , hydrogen peroxide , polyacrylamide gel electrophoresis , dentistry , gel electrophoresis , sodium dodecyl sulfate , anterior teeth , biochemistry , medicine , enzyme
There are numerous bleaching products available to whiten the appearance of teeth: some are done only in the office of a dentist and some can be done by the consumers. There is evidence to show that those products (usually containing hydrogen peroxide) used in dentist's office does result in a decrease in the level of protein as well as the strength of the enamel of teeth. The goal of this project was to evaluate the effect of over the counter whitening agents on teeth by measuring both total protein as well as looking at individual proteins of the teeth. A method to extract protein from teeth was used and assessed and total protein was measured using the Lowry assay. The individual proteins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and the molecular weights of the proteins were compared to those proteins known to be in the enamel or dentin of teeth. These proteins were further characterized based on amino acid composition.