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Characterization of YML020W in Saccharomyces cerevisiae
Author(s) -
Shoemaker Katherine,
Keeney Jill
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1076.5
Subject(s) - saccharomyces cerevisiae , orfs , gene , genetics , open reading frame , genome , biology , model organism , genetic screen , function (biology) , computational biology , mutant , yeast , peptide sequence
Saccharomyces cerevisiae is a single‐celled, eukaryotic yeast commonly known as baker's or brewer's yeast. As a model organism, genes can be easily inserted and removed allowing researchers to utilize deletion genetics to better understand the function of open reading frames (ORFs) within the S. cerevisiae genome. ORFs are characterized using gene ontology, which includes three categories: biological processes, cellular components, and molecular functions. The characterization of genes within the S. cerevisiae genome is of importance in order to understand the functionality of these uncharacterized genes. Through a better understanding of how genes and their products interact with one another, we can gain insight into the function of S. cerevisiae as a model organism. The purpose of this study is to characterize the S. cerevisiae ORF YML020W , which was first identified in a screen for resistance to the apoptosis‐inducing drug, KP1019 . In order to further the understanding of the structure and function of this ORF, a deletion strain of YML020W was constructed. Genetic interactions with DUN1 and YML020W were characterized through the construction of a double deletion mutant. To determine the location of the gene within the cell, the protein product associated with YML020W was fluorescently labeled at the C‐terminus and observed using fluorescence microscopy where clusters of YML020Wp were visible. These strategies will add details to gene ontology and provide information concerning of the importance of YML020W in S. cerevisiae . Support or Funding Information This work was supported by Juniata College, Howard Hughes Medical Institute, and the National Science Foundation