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Gene Expression Profiles of Various Rat Brain Regions Following Exposure to Soman
Author(s) -
Gautam Aarti,
Sowe Bintu,
Donohue Duncan,
Miller StacyAnn,
Hammamieh Rasha,
Meyerhoff James,
Pham Michael,
Lumley Lucille,
Jett Marti
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1076.1
Subject(s) - soman , hippocampus , nerve agent , pharmacology , piriform cortex , brain damage , medicine , chemistry , acetylcholinesterase , neuroscience , toxicology , anesthesia , biology , biochemistry , enzyme
Soman (GD) is a highly toxic man‐made substance and a potential chemical weapon. It belongs to a class of chemicals known as organophosphorus (OP) nerve agents, which may cause seizures, respiratory failure, and death. Soman undergoes aging and is one of the more difficult nerve agent exposures to treat since field available oxime therapy is not effective against soman. Understanding the effects of soman is vital to developing a method of detection and treatment for exposure. Exposure to GD can consequently result in neuropathological abnormalities in various regions of the brain, including the hippocampus, piriform cortex, amygdala, thalamus, and hypothalamus. In this study, rats were exposed to either 0.8 LD 50 GD or 1.0 LD 50 GD versus a saline control group and evaluated for seizure activity. Rats were sacrificed and brain regions were harvested at 72 hours post‐exposure. Brain tissues were homogenized and RNA was isolated using TRIzol reagent. In order to explore the pathways and biological processes associated with GD exposure, transcripts in the aforementioned brain regions were assayed using Agilent's rat gene expression arrays. Detailed analysis is currently underway to compare the expression profile generated from different regions of the brain and to understand the differences in response to the variation in the concentration of GD exposure. DISCLAIMER “Research was conducted in compliance with the Animal Welfare Act and all other Federal requirements. The views expressed are those of the authors and do not constitute endorsement by the U.S. Army.”