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The Effects of Genistein Treatment on the Proximal Femur of Obese Diabetic Mice
Author(s) -
Odle Briton,
Dennison Nathan,
AlNakash Layla,
Broderick Tom L,
Plochocki Jeffrey H
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1039.9
Subject(s) - genistein , endocrinology , medicine , hyperinsulinemia , femur , bone mineral , epiphysis , bone density , streptozotocin , diabetes mellitus , osteoporosis , anatomy , insulin resistance , surgery
Measurements of bone mineral density in obese, type 2 diabetic patients and ob/ob mice show normal, or even elevated, bone density of the lumbar spine, but reduced bone density of compact bone in limbs. In a previous study, we showed that genistein treatment increases bone mass at the midshaft of limb bones of obese diabetic ob/ob mice. In this study, we examine the effects of genistein treatment on bone of the proximal femoral epiphysis of ob/ob mice to determine if similar beneficial effects of genistein are observed in a region of the femur predominated by trabecular bone and not compact bone. Twenty lean control mice and mice of the ob/ob strain that demonstrate severe obesity, hyperinsulinemia, and hyperglycemia were treated with 600 mg/kg genistein or vehicle. Consistent with findings of trabecular bone in the lumbar spine, we find obese, diabetic mice exhibit significantly greater total area (Tt.Ar.) and bone area (B.Ar.) at the proximal epiphysis in comparison to lean controls (P < 0.05). However, the proportion of bone to the total area of the proximal epiphysis (B.Ar./Tt.Ar.) was similar between groups. Genistein treatment had no effect on bone of the proximal femoral epiphysis in lean controls or obese diabetic mice. Our findings suggest the benefits of genistein to bone health in the ob/ob mouse model are limited to compact bone of the femur.Ratio of bone area to total tissue area (B.Ar./Tt.Ar.) of the proximal femur of lean control and obese diabetic mice treated with 600 mg/kg genistein and vehicle.