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Production Of Interleukin 1 Beta In Primary, Serum‐Free Cultures Of Human Osteoarthritic Chondrocytes
Author(s) -
LozanoTorres Xiomara,
Buckley Andrea Lynn,
Miller Lawrence,
Bundens Grace,
Datillo Victoria,
Milton LaBraya,
Behling Kathryn,
Chmielewski Sarah,
Cho Ellen,
Selim Abdulhafez,
GeorgeWeinstein Mindy,
D'Angelo Marina
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1039.7
Subject(s) - cartilage , osteoarthritis , chondrocyte , cytokine , extracellular matrix , pathology , chemistry , femoral condyle , type ii collagen , interleukin , andrology , immunology , medicine , anatomy , biochemistry , alternative medicine
Osteoarthritis (OA) is characterized by a loss of cartilage extracellular matrix (ECM) and an increase of cytokine production in the synovial joint. Notably, Interleukin‐1 beta (IL‐1β), elevated in OA cartilage, induces a number of responses from chondrocytes, including release and activation of matrix metalloproteases (MMPs). Enhanced degradation of the ECM, a hallmark of OA pathology, is likely due, in part, to increased cytokine production. In this study, osteoarthritic human chondrocytes isolated from total knee arthroplasty were reared in three‐dimensional, serum‐free, alginate cultures. The sides of least and greatest pathology of the femoral condyles and tibial plateau were determined by gross inspection in the surgery suite and histological assessment. Medial and lateral cells isolated from femoral condyles and tibial plateau were cultured separately. Chondrocytes were plated at 2.5×10 6 cells per ml of alginate and incubated in 2 milliliters of serum‐free medium for five days. Conditioned media was collected on days 2 and 5 of culture, concentrated by centrifugation and IL‐1β measured by immunoassay. IL‐1β production was detected in medial and lateral chondrocyte cultures; however, the concentration of this cytokine was highest in the cultures established from the region of greatest pathology. Collagens type I and II degradation products were also increased in cultures of cells from the side of greatest pathology. These results demonstrate a correlation between elevated levels of IL‐1 β, collagen degradation and extent of pathology in primary cultures that maintain the phenotype of human articular chondrocytes. This culture system provides a platform with which to further define the feedback loop between chondrocyte produced cytokines and MMPs in OA. Support or Funding Information Center for Chronic Disorders of Aging Pilot Grant, PCOM