Premium
The Effects of Staphylococcus aureus Biofilm Conditioned Medium on Fibroblast Tension Generation and Migration
Author(s) -
Kshetri Pratiksha,
Brennan Robert E,
Vaughan Melville B
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1034.9
Subject(s) - biofilm , staphylococcus aureus , wound healing , microbiology and biotechnology , fibroblast , myofibroblast , cell migration , contraction (grammar) , chemistry , cell , bacteria , biology , immunology , in vitro , medicine , pathology , biochemistry , fibrosis , genetics , endocrinology
Chronic wounds are often characterized by persistent inflammation. These wounds provide a favorable environment for bacteria to establish an infection, which may lead to the formation of a biofilm that can delay the healing process. Although the occurrence of biofilms in chronic wounds is known, the role of these biofilms in chronic wound pathogenesis is still unclear. Chronic wounds may be affected directly by the biofilm or through interaction with secreted products of the biofilm bacteria. Our goal was to investigate the Staphylococcus aureus biofilm extract effects in collagen lattice tension generation on fibroblasts. The control and TGF‐beta treated lattices were cultured in presence or absence of S. aureus biofilm conditioned medium. The mature control lattices showed the greatest contraction followed by biofilm with less contraction. The reduction of contraction was correlated with less number of proliferated and differentiated myofibroblasts in biofilm‐conditioned treated lattices. Tension generation is one of the important factors in wound closing by myofibroblasts; cell migration is another important aspect of wound healing. We also hypothesized that the biofilm conditioned medium of S. aureus would affect the migration of fibroblasts. The quantitative analysis of two‐dimensional cell migration was conducted using silicone elastomer mask migration assay. Cells were cultured in the presence or absence of biofilm conditioned medium and the migratory response was measured with the image of cell free area invaded by fibroblasts at T0, T24, T48 and T72 hrs. The percentage of area invaded by fibroblasts in control at T48 was found to be 62.04 whereas the percentage of area invaded for biofilm‐treated cells was 58.05. In conclusion, fibroblast tension generation and migration behavior was inhibited by the biofilm conditioned medium. The future goal is to investigate the mechanism behind the inhibition properties of biofilm conditioned media on fibroblasts. Support or Funding Information 3M Corporation and UCO Office of Research and Grants