Premium
The Baylor KOMP2 Program and BaSH Consortium: A Resource for Producing and Phenotyping Knockout Mice
Author(s) -
Reynolds Corey L,
Beaudet Arthur,
Dickinson Mary,
Seavitt John,
Heaney Jason
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1028.1
Subject(s) - knockout mouse , biology , crispr , embryonic stem cell , phenotype , conditional gene knockout , gene knockout , laboratory mouse , bioinformatics , mouse strain , computational biology , gene , genetics
The Knockout Mouse Phenotyping Program (KOMP2) was launched in the Fall of 2011 with the goal of having three mouse production and cryopreservation centers generate a knockout mouse strain for every protein coding gene by using embryonic stem cell resources generated by the International Knockout Mouse Consortium (IKMC). In addition to the generation of these knockout lines of mice, each center is responsible for the systematic, broad‐based, phenotyping of each line using standardized procedures. Phenotyping procedures start when the animal is nine weeks of age and conclude at sixteen weeks of age. The tests include behavioral, metabolic, cardiovascular, auditory, ocular, and histopathological assays. Gene‐to‐phenotype associations are made by a versioned statistical analysis with all data (quantitative and images) freely available on a web portal which offers several data download features. New technology development has been supplemented throughout the projects lifespan to include the standardized phenotyping of embryos from embryonic lethal mouse lines, usage of CRISPR/Cas9 technology to increase productivity and a subset of lines will be examined for small molecule metabolites to detect metabolic abnormalities. At present, the KOMP2 project has produced over 2,500 lines of mice and phenotyping of these lines is on track to be completed by Fall 2016 with all data currently being freely available to the scientific community. Support or Funding Information NIH U54‐HG006348