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Role of renal nerves in cardiovascular nuclei and kidneys in renovascular hypertensive rats
Author(s) -
Nishi Erika E,
Gomes Guiomar N,
Perry Juliana C,
Simões Alex Y,
Bergamaschi Cassia T,
Campos Ruy R
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1006.14
Subject(s) - kidney , hydralazine , medicine , renovascular hypertension , endocrinology , plasma renin activity , rostral ventrolateral medulla , denervation , blood pressure , renal medulla , renin–angiotensin system , medulla , renal artery , hypothalamus , nephrectomy , heart rate
Our aim was to investigate whether renal nerves play a role in the renal injury process and modulation of AngII receptors expression in the central nuclei and kidneys in renovascular hypertension. Denervation of clipped kidney (RD: visible bundles were dissected + 10% phenol) was performed in Wistar male rats (n=5/group) 4–5 weeks after clip implantation (gap width: 0.2mm). Ten days after RD, blood pressure (BP) was significantly reduced in the 2K‐1C rats. Gene expression of upregulated AT1R and AT2R within the rostral ventrolateral medulla (RVLM) and paraventricular nucleus of the hypothalamus (PVN) in the 2K‐1C group was normalized after RD. Following RD, there was no change in plasma renin activity in control and 2K‐1C rats. In order to distinguish the effects of RD from the reduction in BP, 2K‐1C rats were treated orally with hydralazine (25mg/kg/day for seven consecutive days). Hydralazine significantly reduced BP and AT1R and AT2R within the RVLM and PVN in the 2K‐1C rats. Renal injury was observed in both ischemic and contralateral kidneys of 2K‐1C rats. Following RD, renal injury was attenuated in both kidneys of hypertensive rats, however, hydralazine improved injury only in the contralateral kidney. These results demonstrate that the renal nerves contribute to AngII receptors changes within the cardiovascular nuclei and renal injury in the ischemic kidney independent of plasma renin activity in the 2K‐1C model of arterial hypertension. Support or Funding Information Supported by FAPESP and CNPq.