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Association of a lipoprotein‐like particle with bovine fetuin
Author(s) -
Kumbla Lakshmikantha,
Cayatte Antonio J.,
Subbiah M. T. Ravi
Publication year - 1989
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.3.9.2472993
Subject(s) - fetuin , chromatography , chemistry , agarose , gel electrophoresis , sodium dodecyl sulfate , lipoprotein , ultracentrifuge , biochemistry , cholesterol , glycoprotein
Fetuin is a major protein of fetal bovine serum that exhibits heterogeneity and has been found to be associated with some biological active growth factors. Preliminary studies indicated that commercial fetuin preparations contain lipids. We investigated in detail the nature of lipids associated with fetuin by using ultracentrifugation and agarose gel chromatography followed by lipid analysis. Fetuin was associated with a variety of lipids, predominantly cholesterol, cholesteryl ester with smaller amounts of phospholipids, triglycerides, and free fatty acids. Adjustment of fetuin preparation for various densities followed by ultracentrifugation resulted in a fraction with a density 1.063‐1.21 g/ml (1‐2% of total protein) that contained the bulk of the lipids. This fraction eluted as a single peak upon high pressure liquid chromatography and agarose gel chromatography. Delipidation of the lipoprotein‐like particle followed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis indicated a major band in the range of fetuin itself. These studies suggest that a fraction of fetuin (or isoform) binds lipids forming a particle with floating characteristics similar to high density lipoproteins.— K umbla , L.; C ayatte , A. J.; S ubbiah , M. T. R. Association of a lipoprotein‐like particle with bovine fetuin. FASEB J. 3: 2075‐2080; 1989.