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Promoter specificity and modulation of RNA polymerase II transcription
Author(s) -
Saltzman Alan G.,
Weinmann Roberto
Publication year - 1989
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.3.6.2649403
Subject(s) - transcription factor ii d , rna polymerase ii , rna polymerase ii holoenzyme , transcription factor ii f , general transcription factor , transcription (linguistics) , microbiology and biotechnology , rna polymerase , biology , transcription factor ii b , polymerase , transcription factor ii e , rna polymerase i , promoter , rna dependent rna polymerase , gene , gene expression , rna , genetics , linguistics , philosophy
RNA polymerase II is a multisubunit enzyme involved in the transcription of protein encoding genes. Recently acquired knowledge of the transcription process and of the RNA polymerase molecule as well as the isolation of subunit clones have led to a better understanding of the enzyme's functional regulation. Specific transcription initiation occurs at promoter regions located upstream of the gene and requires a minimum of five basic factors in addition to the enzyme. Furthermore, proteins that bind to specific DNA elements within the promoter also regulate transcriptional activity. Additional factors are required for the elongation and, possibly, termination of transcription. Two elongation factors, SII and TFIIF, interact directly with the RNA polymerase II molecule. Functional domains of RNA polymerase II have been determined by analysis of genomic clones for the two largest subunits of the enzyme. For example, the 240‐kDa largest subunit contains a highly phosphorylated carboxyl‐terminal heptapeptide domain repeated 26‐52 times that is absolutely required for transcription in vivo. Analysis of the polymerase molecule and its interaction with basic gene‐specific transcription factors will aid in our studies of the control of gene expression.— S altzman , A. G.; W einmann , R. Regulation of RNA polymerase II activity. FASEB J. 3: 1723‐1733; 1989.