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Oxalyl thiolester concentrations decreased in lectin and phorbol ester‐stimulated lymphocytes
Author(s) -
Skorczynski Stephen S.,
Mastro Andrea M.,
Hamilton Gordon A.
Publication year - 1989
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.3.12.2792647
Subject(s) - phorbol ester , lectin , chemistry , microbiology and biotechnology , phorbol , biochemistry , biology , signal transduction , protein kinase c
Oxalyl thiolesters (OTEs) are a newly discovered class of mammalian metabolites that are believed to function in controlling animal metabolism and possibly serve as intracellular mediators for some hormones. Previous correlations had suggested that the concentrations of OTEs might be decreased when cells are stimulated to proliferate, and in our research that was found to be the case. Thus, when bovine lymph node lymphocytes are stimulated either with concanavalin A (Con A) or with a combination of phytohemagglutinin (PHA) and the phorbol ester 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA), the concentration of OTEs in the lymphocytes decreases within 3 h by a factor of approximately two. With either PHA or TPA alone, the decrease in OTE concentration is considerably smaller. With Con A as stimulant, the OTE levels decrease within 1 h and remain low for at least 24 h. It was also noted that the concentration of OTEs in unstimulated isolated lymphocytes is significantly lower in lymphocytes obtained from 2‐year‐old animals than in lymphocytes obtained from older animals. The results of the current investigation, when considered in conjunction with other recent results, suggest that OTEs may be natural cell proliferation inhibitors.—S korczynski , S. S.; M astro , A. M.; H amilton , G. A. Oxalyl thiolester concentrations decreased in lectin and phorbol ester‐stimulated lymphocytes. FASEB J. 3: 2415‐2419; 1989.