Premium
Occurrence of platelet‐activating factor (PAF) in normal rat stomach and alteration of PAF level by water immersion stress
Author(s) -
Sugatani Junko,
Fujimura Kazuyo,
Miwa Masao,
Mizuno Takako,
Sameshima Yoshiko,
Saito Kunihiko
Publication year - 1989
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.3.1.2910739
Subject(s) - platelet activating factor , antrum , stomach , endocrinology , medicine , serotonin , chemistry , platelet , endogeny , receptor , biological activity , biology , biochemistry , in vitro
We detected platelet‐activating substance in gastrointestinal areas, which was confirmed to be platelet‐activating factor (PAF) on the basis of the following findings: 1 ) it comigrated with authentic PAF on thin‐layer chromatography; 2 ) it did not aggregate PAF‐desensitized platelets; and 3 ) its activity was completely antagonized by the receptor antagonists CV3988 and L‐652,731. The level of PAF was determined with a bioassay method based on the release of [ 3 H] serotonin from washed rabbit platelets. In the normal rat stomach, the level of PAF was high in the antrum (940 ± 200 nmol PAF/mol phosphorus of original phospholipids), especially in the antral mucosa (1801 ± 426 nmol/mol phosphorus of original phospholipids). The stomach PAF level was significantly altered by water immersion stress. Stress for a period of 1 h was associated with a decrease in the antral PAF level to 39 ± 7% of that of untreated controls. This low PAF level persisted during stress. On the other hand, in the corpus, stress for periods of 1 and 3 h was associated with decreases in the PAF content, and further stress (7 h) resulted in restoration of the PAF level to normal. Furthermore, 7 h of stress was associated with distinct hemorrhagic lesions, which were prevented by CV3988 infused i.v. before the stress. This is the first report of an association between a decrease of the endogenous PAF level in animal tissues and tissue damage.—S ugatani , J.; F ujimura , K.; M iwa , M.; M izuno , T.; S ameshima , Y.; S aito , K. Occurrence of platelet‐activating factor (PAF) in normal rat stomach and alteration of PAF level by water immersion stress. FASEB J. 3: 65‐70; 1989.