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Nuclear disintegration of target cells by killer B lymphocytes from tumor‐bearing mice
Author(s) -
Lopez Diana M.,
Blomberg Bonnie B.,
Padmanabhan Ranga R.,
Bourguig Lilly Y. W.
Publication year - 1989
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.3.1.2783411
Subject(s) - antibody dependent cell mediated cytotoxicity , microbiology and biotechnology , cytotoxicity , antibody , effector , receptor , cytolysis , fc receptor , cytoplasm , lytic cycle , biology , chemistry , immunology , in vitro , monoclonal antibody , biochemistry , virus
Normal murine B lymphocytes are not known to be effectors of the Fc receptor‐mediated, antibody‐dependent cellular cytotoxicity (ADCC). In contrast, we report here that highly purified splenic B cells from mammary tumor‐bearing mice develop the potential of lysing antibody‐coated target cells. These lymphocytes are characterized by being G‐10 nonadherent, nylon wool adherent, sIg ∗ , FcR + , Thy 1.2 − , asialo GM 1 − , and the immunoglobulin heavy‐chain genes of both chromosomes are rearranged. The lytic reaction is characterized by a noninterdigitating binding and by the appearance of endocytotic vesicles in the target cells. Nuclear disintegration occurs 18 h after initial effector‐target cell conjugate formation. At such time, only minor cytoplasmic membrane alterations are evident. The emergence of killer B cells in tumor‐bearing hosts indicates that all lymphoreticular cell types bearing Fc receptors are capable of mediating ADCC.— L opez , D. M.; B lomberg , B. B.; P admanabhan , R. R.; B ourguignon , L. Y. W. Nuclear disintegration of target cells by killer B lymphocytes from tumor‐bearing mice. FASEB J. 3: 37‐43; 1989.