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Antagonism of corticotropin releasing factor (CRF) in the locus coeruleus (LC) increase respiratory response to hypercapnia
Author(s) -
Incheglu Jolene,
Silva Glauber,
Bicego Kenia,
Gargaglioni Luciane
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb742
Subject(s) - hypercapnia , normocapnia , endocrinology , medicine , antagonist , chemoreceptor , respiratory system , tidal volume , receptor , chemistry , locus coeruleus , ventilation (architecture) , anesthesia , central nervous system , mechanical engineering , engineering
LC is a noradrenergic pontine group that acts as a central chemoreceptor to CO 2 /pH and it is involved in the cognitive aspects of stress response, in part through the action of CRF. Thus, we tested the involvement of CRF1 receptors (CRF1) located in the LC in the ventilatory and thermal responses induced by hypercapnia (7%CO 2 ) in rats. To this end, we injected antalarmin (a CRF1 antagonist, 0.05 µg/0.1 µL) into the LC of male Wistar rats. Pulmonary ventilation (V E ) and body temperature (Tb, dataloggers) were measured in air and followed by 7% CO 2 in unanesthetized rats. V E and Tb were not different between groups under normocapnia. The hypercapnic ventilatory response of CRF1 antagonist treated animals was 23.2% higher at 15 min after CO 2 exposure compared with vehicle animals (CRF1: 2166.3 ± 206.6 vs vehicle: 1664.3 ± 155.9), due to an increased tidal volume. Our results suggest that CRF1 receptors in the LC contribute to the hypercapnic chemoreflex control in unanesthetized rats. Financial Support: FAPESP and CNPq.