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Genetic Regulation of Endothelial Responses to Exercise Training
Author(s) -
Kim Seung,
Avila Joshua,
Massett Michael
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb728
Subject(s) - sed , sodium nitroprusside , strain (injury) , high intensity interval training , interval training , snp , endothelium , medicine , endocrinology , inbred strain , zoology , biology , chemistry , genetics , nitric oxide , genotype , single nucleotide polymorphism , gene
Exercise training improves endothelial function in a duration and intensity‐dependent manner. The aim of this study was to characterize the genetic contribution to phenotypic variation in endothelial adaptation to exercise training. Concentration response curves to acetylcholine (ACh) and sodium nitroprusside (SNP) in isolated thoracic aortas from male mice (n ∼ 6/group/strain, 12 wks) from 26 inbred strains were obtained after moderate intensity continuous training (MOD) or sedentary controls (SED) for 4 weeks. Endothelium‐dependent relaxation to ACh (EDR) in SED mice varied by ~2 fold between two strains having the highest and lowest responses. However, only A/J and NON showed greater EDR in MOD mice compared to SED. Responses to SNP were similar among strains and between groups. These results imply that MOD for 4 weeks might not be sufficient to stimulate changes in endothelial function. Thus, a follow‐up study was conducted to examine the effects of different training modes (intensity and duration) on EDR in 4 selected inbred strains (NON, 129S1, C57BL6, SJL). Male mice from each strain were assigned into 6 groups (n = 6/group): SED, MOD or high intensity interval training for 4 or 8 weeks. Concentration response curves to ACh and SNP were measured after training. There was a significant interaction between strain and training mode for EDR. In 129S1 and C57BL6 mice, exercise training had no effect on EDR. EDR in NON was greater in 4 wk‐trained mice of either intensity, but not 8 wk, compared with SED. Interestingly, EDR was lower in 4wk‐high intensity interval trained‐SJL compared to SED. Responses to SNP were similar across all mice. These data indicate that there is a complex interactive effect between genetic background and training mode on endothelial function. Supported by NIH grant HL085918 to MPM